Orally administered docetaxel-loaded chitosan-decorated cationic PLGA nanoparticles for intestinal tumors: formulation, comprehensive in vitro characterization, and release kinetics

• Sedat Ünal,
• Osman Doğan and
• Yeşim Aktaş

Beilstein J. Nanotechnol. 2022, 13, 1393–1407, doi:10.3762/bjnano.13.115

• and free DCX were carried out using the Caco-2 cell line. Drug molecules have to overcome several barriers throughout the GIT, including the mucus layer, intestinal epithelial cells, and the endothelium of the capillaries. Among them, the monolayer of epithelial cells plays an essential role in the

Low uptake of silica nanoparticles in Caco-2 intestinal epithelial barriers

• Dong Ye,
• Mattia Bramini,
• Delyan R. Hristov,
• Sha Wan,
• Anna Salvati,
• Christoffer Åberg and
• Kenneth A. Dawson

Beilstein J. Nanotechnol. 2017, 8, 1396–1406, doi:10.3762/bjnano.8.141

• capacity of nanoparticles to enter and transport across such barriers. In this work, Caco-2 intestinal epithelial cells were used as a well-established model for the intestinal barrier, and the uptake, trafficking and translocation of model silica nanoparticles of different sizes were investigated using a

Atomic force microscopy as analytical tool to study physico-mechanical properties of intestinal cells

• Christa Schimpel,
• Oliver Werzer,
• Eleonore Fröhlich,
• Gerd Leitinger,
• Markus Absenger-Novak,
• Birgit Teubl,
• Andreas Zimmer and
• Eva Roblegg

Beilstein J. Nanotechnol. 2015, 6, 1457–1466, doi:10.3762/bjnano.6.151

• secreted by goblet cells and forms an efficient acellular barrier that strongly impacts adhesive interactions between intestinal epithelial cells and diverse substances/antigens. Due to intake of food, differences in the pH occur, which leads to changes in the viscoelastic properties of the mucus layer

Coating with luminal gut-constituents alters adherence of nanoparticles to intestinal epithelial cells

• Heike Sinnecker,
• Katrin Ramaker and
• Andreas Frey

Beilstein J. Nanotechnol. 2014, 5, 2308–2315, doi:10.3762/bjnano.5.239

• gut-constituents on the adherence of nanoparticles to intestinal epithelial cells. Carboxylated polystyrene particles 20, 100 and 200 nm in size represented our anthropogenic NPs, and differentiated Caco-2 cells served as model for mature enterocytes of the small intestine. Pretreatment with the

• increasing colloid stability or camouflaging attachment sites, certain components of intestinal fluid are capable to modify particle surfaces in such a way that interactions with cellular surface structures result in an increased binding. Keywords: adherence; agglomeration; intestinal epithelial cells

• intestinal epithelial cells we used the human colorectal adenocarcinoma cell line Caco-2 as model enterocytes because Caco-2 cells can spontaneously differentiate into a columnar epithelium after reaching confluence [19] and because the morphological and biochemical features of differentiated Caco-2 cells

The gut wall provides an effective barrier against nanoparticle uptake

• Heike Sinnecker,
• Thorsten Krause,
• Sabine Koelling,
• Ingmar Lautenschläger and
• Andreas Frey

Beilstein J. Nanotechnol. 2014, 5, 2092–2101, doi:10.3762/bjnano.5.218

• to each other by tight junctions [22]. The epithelial turnover of the enterocytes provides an additional type of hurdle [23] as NP-ladden intestinal epithelial cells would be sloughed off and be excreted within a few days. For the uptake of supramolecular entities, in particular microbial foes